Red Routes are now enforceable on certain roads in Brighton, meaning vehicles cannot stop on them apart from in designated bays.
Restrictions came into force on London Road, Preston Road and Lewes Road seven days a week on Monday.
On its website, Brighton & Hove City Council (BHCC) said the Red Routes would help "tackle anti-social parking and to keep traffic in the city moving".
Roads are marked with double red lines, instead of double yellow lines, and fines can be issued to road users who break the restrictions.
A white road sign with red borders on a street in Brighton, it reads "red route, no stopping at any time, except loading only"
"On Red Routes you can only stop, park and load or unload in specially marked bays," BHCC said on its website.
A penalty charge notice for stopping is £70, reduced to £35 if paid within 14 days.
Blue Badge holders can be picked up or dropped off if the vehicle is not parked, and taxis can stop for passengers.
Emergency services and waste collection services are exempt from the restrictions, but deliveries can only stop in designated loading bays.
The new restrictions are causing concerns for local businesses, some of which fear that their businesses will be negatively impacted.
A middle aged man wearing a cap and royal blue butchers coat and blue and white striped apron smiles at the camera in front of his butchers shop in Brighton
Butcher Graham Maides who runs M & B Meats on London Road told BBC Radio Sussex:
"It's going to be devastating. I'm getting four deliveries per morning with 17 to 20 boxes coming across to me and if the loading bays are full I don't know where to suggest they
go.
"The chances are that I will lose a day's meat so that's trade I'm losing. We've got to get some more loading bays because in this area every shop has no back entrance so everything has to come through the front."
The council said the Red Routes would help improve safety for pedestrians, cyclists, people using buses and drivers.
The red route in London Road begins at Cheapside and joins Preston Road, stopping at South Road. In Lewes Road it is between Melbourne Street and Elm Grove.